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KMID : 1134820150440070975
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Journal of the Korean Society of Food Science and Nutrition
2015 Volume.44 No. 7 p.975 ~ p.982
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Induction of Apoptosis by Piceatannol in YD-15 Human Oral Cancer Cells
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Lee Hae-Nim
Jang Hye-Yeon
Kim Hyeong-Jin
Shin Seong-Ah
Choo Gang-Sik
Park Byung-Kwon
Kim Byeong-Soo
Jung Ji-Youn
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Abstract
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Piceatannol (trans-3,4,3',5'-trihydroxystilbene), a natural stilbene, is an analogue of resveratrol. In the present study, possible mechanisms by which piceatannol exerts its pro-apoptotic action in cultured human oral cancer YD-15 cells were investigated. To investigate whether or not piceatannol has effects on cancer cell viability, human oral YD-15 cells were treated with piceatannol (0, 50, and 100 μM). Piceatannol treatment (100 μM) showed the strongest inhibition of cell proliferation and reduced cell viability in a dose-dependent manner. Chromatin condensation detected by DAPI staining significantly increased in a concentration-dependent manner, indicating apoptosis. Piceatannol treatment activated initiator Bax (pro-apoptotic) and cPARP in a concentration-dependent manner. Further, piceatannol induced down-regulation of Bcl-2 (anti-apoptotic). We also evaluated the activity of piceatannol against oral cavity cancer tumors in mice. Piceatannol-treated nude mice bearing YD-15 xenograft tumors exhibited significantly reduced tumor volume and weight due to the potent effect of piceatannol on tumor cell apoptosis, as determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Immunohistochemistry staining showed elevated expression of cleaved-caspase-3 as well as reduced expression of Ki-67 in the piceatannol-treated group. Therefore, piceatannol can be developed as a cancer preventive medicine due to its growth inhibitory effects and induction of apoptosis in human oral cancer cells.
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KEYWORD
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piceatannol, oral cancer cell, apoptosis, anticancer
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